English: Correlative microscopy of thin sections of Cyanothece sp. ATTC 51142 cells

Note: This is now Crocosphaera subtropica strain ATCC 51142, see NCBI: https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?id=43989 and LPSN: https://lpsn.dsmz.de/species/crocosphaera-subtropica

Shown are examples of TEM images (A–C) and the corresponding nanoSIMS images (D–F). The dashed polygon in panels (D–F) shows the boundary of the TEM image aligned within the NanoSIMS image. Shown are images from samples collected at 09:30 following a morning incubation (07:30–09:30) from the N2 culture (A,D) and NO3 culture (B,E), and from a sample collected at 07:00 following a night-time incubation (21:45–07:00) from the NO3 culture (C,F). Arrows in the images point to sub-cellular structures identified as carboxysomes (c), cyanophycin inclusions (cy), polyphosphate bodies (p), polysaccharide granules (ps), thylakoid membranes (t), and nucleoid (n). NanoSIMS images are shown as RGB overlays of secondary ion counts 12C14N– (red), 32S– (green), and 31P– (blue). Note that the hues are not comparable among the images because, for each overlay, the contrast for the three color channels was modified so as to enhance the overall visibility of the intracellular variability. In addition to P–, the polyphosphate bodies had markedly increased O– ion counts (data not shown).